FastQCFastQC Report
Sun 14 Apr 2019
SRR6576057_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576057_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773174
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15820.20461112246402494No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13100.16943146044745427No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11540.14925488958500932No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10890.1408479850589906No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10330.13360511346734372No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10140.13114771060589206No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9790.12662091586111276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.11963671825488184No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA9240.11950738126217383No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8950.11575660847364241No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8680.11226450967052695No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8630.11161782470698704No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8100.10476296409346408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.067.591171
GTATCAA24350.065.5987241
ATCAACG34650.046.1213073
TCAACGC35100.045.6639254
CAACGCA35250.045.3362665
AACGCAG36100.044.5291986
TATCAAC37750.042.3338622
ACGCAGA42150.038.0212677
CGCAGAG43100.037.183218
GCAGAGT45600.034.835479
GTACATG52950.029.0988921
ACATGGG53200.028.0958023
TACATGG54650.028.038412
ACCTAAG5250.027.8225571
TAATACC856.811479E-427.6488484
AGAGTAC43950.027.32132710-11
TAGTACT1904.474714E-1027.2122864
GTGCAAG3050.026.2629641
CATGGGA28500.026.0578254
GGTAATC2357.2759576E-1225.998328
Produced by FastQC (version 0.11.2)