FastQCFastQC Report
Sun 14 Apr 2019
SRR6576055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169829
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16560.14155915095283156No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14920.1275400079840729No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14700.12565939124436135No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14620.12497553061173898No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13820.11813692428551523No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13750.11753854623197066No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13460.11505955143871455No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12360.1056564677401569No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11880.10155330394442263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20700.059.502481
GGTATCA6700.058.238131
TCAACGC26300.045.748514
ATCAACG27250.044.1536063
CAACGCA28400.043.358645
AACGCAG29300.042.026816
TATCAAC33350.036.7916072
ACGCAGA36150.033.9332127
CGCAGAG36800.033.3338478
TAGGTAT4850.031.9790025
GTACATG48850.031.8542611
GTCTTAG6750.030.6445331
CATATAG1859.094947E-1230.4861263
AGGTATA5100.030.4114046
TACATGG50700.030.3210262
GCAGAGT40550.030.2511839
ACATGGG51000.029.8584693
TTAGGTA5300.029.2638054
GGTATAG5200.028.9227357
CTTAGGT5200.028.9227353
Produced by FastQC (version 0.11.2)