FastQCFastQC Report
Sun 14 Apr 2019
SRR6576054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887713
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23040.2595433434004008No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16770.188912407501073No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14150.15939836411092323No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13120.1477955149918949No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11170.12582895598014224No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10980.1236886245892535No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9460.10656597346214373No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA9130.10284855578323174No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9000.10138411851578157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21700.054.432471
ATCAACG28000.041.1233983
CAACGCA28300.040.687465
GGTATCA8250.040.4993821
TATCAAC31500.037.600652
AGGTATA4650.036.3836336
TAGGTAT4800.036.2277535
AACGCAG32200.036.195316
TCAACGC32600.035.4648744
CGCAGAG33500.034.2488678
ACGCAGA33600.034.1238567
TTAGGTA5150.033.7656754
CTTAGGT5100.033.175183
GCAGAGT36300.031.7366189
TAGTACT5750.029.424944
GTACATG44600.029.2273181
GTCTATA1452.6264388E-829.2091241
ACATGGG44750.028.5664833
CTGTGCG6750.028.5615169
TACATGG45850.028.2927472
Produced by FastQC (version 0.11.2)