FastQCFastQC Report
Sun 14 Apr 2019
SRR6576054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887713
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22040.24827844134309177No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17350.19544605069431223No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14290.1609754503989465No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14140.15928571509035017No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12200.13743180509917058No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11410.12853253247389643No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11200.1261669030418615No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11020.12413922067154588No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA9330.10510153619469355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.055.5399631
TACGCGT405.6832976E-446.9877785
GGTATCA8250.043.3003431
ATCAACG29300.040.2523273
CAACGCA29750.039.643475
GGTAATC2150.039.3386048
ACGCGTA500.001703994237.590226
AACGCAG33450.036.803586
TATCAAC33750.035.655162
TCAACGC33650.035.3281064
ACCTAAG8700.034.0371361
TAGTACT4750.033.6333544
CGCAGAG36000.032.760928
ACGCAGA36100.032.6701747
AAGGGTA2750.032.4642835
CTAAGAC10250.032.089223
CGTAGAC753.2799438E-431.3251843
CTGTGCG5250.031.323429
TAAGACA10900.030.6067184
GTACTAG3700.030.4888631
Produced by FastQC (version 0.11.2)