FastQCFastQC Report
Sun 14 Apr 2019
SRR6576050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354952
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17420.1285654399565446No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16200.11956143095843985No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16200.11956143095843985No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15200.11218109571409171No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14890.1098931917883438No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14770.109007551559022No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14500.10701486104304801No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14420.10642443422350016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20100.061.731561
GGTATCA5950.058.4539831
ATCAACG28000.043.967373
TCAACGC28000.043.7995534
CAACGCA29150.042.071615
AACGCAG30150.040.832056
TATCAAC34650.035.809652
ACGCAGA37600.032.8666237
GTACATG53700.031.3334451
ACCGTAT600.004166435531.3253488
ACATGGG55100.030.273593
CGCAGAG41300.029.9221558
TACATGG55850.029.8747712
GCAGAGT44950.027.8050149
GTATAGA1151.3773529E-424.5218241
AGAGTAC38550.024.01115410-11
CATGGGG32700.023.8532494
GGTACCT8300.023.7770738
CATGGGT14250.023.0818374
GTCTTAG4200.022.381031
Produced by FastQC (version 0.11.2)