FastQCFastQC Report
Sun 14 Apr 2019
SRR6576049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523262
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17710.1162636499827344No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15980.10490644419673043No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15900.10438125548986321No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15590.10234614925075265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.060.7250251
GTATCAA23350.056.9243661
ATCAACG30350.042.8986363
TCAACGC30400.042.3628464
CAACGCA31850.040.5791475
AACGCAG33550.038.3828936
TATCAAC37550.035.1737632
ACGCAGA40650.031.7944857
CGCAGAG45250.028.4584798
GTACATG56000.028.1121771
ACATGGG57400.027.1862073
TACATGG57750.027.1842212
ACCTAAG2104.7293724E-1126.933821
GCAGAGT49250.026.6242649
TGTACTA2451.2732926E-1124.9377295
ATAGGGT1551.5465557E-624.2594383
AGAGTAC41500.024.12188110-11
CTATTAA3900.022.9628091
CATGGGG34350.021.8928054
GTCCAAT3059.094947E-1221.6353631
Produced by FastQC (version 0.11.2)