FastQCFastQC Report
Sun 14 Apr 2019
SRR6576044_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576044_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993119
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17480.17601113260344428No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13430.13523052121649068No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12150.12234183416086088No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11800.1188175837940871No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11230.11307809033962697No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10880.1095538399728532No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10770.10844621842901No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10270.10341157504790463No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10260.10331088218028253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16350.061.9858631
GGTATCA5500.059.9939271
TCAACGC25000.040.6104134
ATCAACG25400.039.785833
CAACGCA25950.039.3048445
AACGCAG26850.038.3374756
TATCAAC30200.033.6178932
ACGCAGA32100.032.212137
GTCTTAG5000.032.0538981
GTACATG43650.030.7774451
ACATGGG44850.029.9727973
TACATGG45050.029.6310622
CGCAGAG35400.029.3405978
GCAGAGT37150.028.2114899
GGTAATC1704.0763553E-927.6459228
AGGTATA4450.027.4623056
AGAGTAC32800.026.29312110-11
TAGGTAT4500.026.1126635
TTAGGTA5350.025.478154
CTTAGGT4800.025.4598463
Produced by FastQC (version 0.11.2)