FastQCFastQC Report
Sun 14 Apr 2019
SRR6576042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001586
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC29180.29133793803028396No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT21500.21465954995377332No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC17880.17851687224062637No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT15700.15675139229182516No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA15630.15605250073383614No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12900.128795729972264No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12350.12330443915949305No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA12270.12250570595036271No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC12200.1218068143923737No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT12030.12010950632297177No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11800.11781314834672209No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT11540.11521726541704858No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC10910.1089272413951473No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10880.10862771644172342No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10870.10852787479058214No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC10850.10832819148829954No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10360.10343595058237634No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10340.10323626728009377No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10060.10044070104813765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.050.3153271
CAACGCA31200.039.921615
ATCAACG32200.039.2676473
GTACTAG6700.036.5924721
AACGCAG37200.035.7569356
ACGCAGA35850.034.4778377
CGCAGAG36250.033.8397878
TATCAAC39600.033.116732
TCAACGC39250.032.3342364
GCAGAGT38050.032.1154339
CTTAAGA1052.0635089E-631.3362642
ATAAGCG1052.0635089E-631.3362643
GGTATCA12350.030.5412121
TAGGCAT8800.029.9103955
ACCGTCC804.7806275E-429.3748138
ATAAGGT5300.029.2668863
ACCTAAG2101.8189894E-1229.1868531
ACATGGG41950.029.1326393
AGGGCTA5350.028.9919175
CTATTGA5200.028.9228949
Produced by FastQC (version 0.11.2)