FastQCFastQC Report
Sun 14 Apr 2019
SRR6576040_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576040_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719060
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC22530.313325730815231No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC17210.23934024977053375No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT16820.23391650209996384No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA15770.21931410452535252No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT13910.19344700025032682No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA13310.18510277306483464No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11140.1549244847439713No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11070.15395099157233055No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC10160.14129558034100076No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9900.13767974856062082No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9600.13350763496787474No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC9440.13128250771841016No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC9240.1285010986565794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.12196478736127724No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8560.11904430784635497No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT8470.11779267376853114No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC8460.1176536033154396No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT7960.11070008066086279No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT7860.10930937612994743No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT7380.1026339943815537No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT7310.10166050120991295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20800.048.9587631
CAACGCA23750.040.767375
ATCAACG24350.040.1516763
TATAGTG651.412748E-436.1547855
GTACTAG6100.035.5523221
TATCAAC29250.035.0324022
AACGCAG29850.034.955686
ACGCAGA28150.034.7267277
CGCAGAG28750.034.0019958
CATAAGT702.1838595E-433.57234
GCAGAGT29350.032.9866379
TAGGCAT7300.032.8364685
TCAACGC29800.032.333054
TAGTACT7450.032.175334
GTACATA600.00409750231.4303171
TATTCTC2900.030.7939015
TACTGTG8100.030.7517437
GGTATCA9850.030.6325911
CTAGGCA8250.029.0552984
GTACATG31600.028.9437391
Produced by FastQC (version 0.11.2)