FastQCFastQC Report
Sun 14 Apr 2019
SRR6576036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424222
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5850.13789949601859405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.044.2384341
CGTTCGT450.001013319241.7770465
TCAACGC12450.034.7303124
ATCAACG12650.034.5527533
CAACGCA12650.034.1812175
AACGCAG12800.033.7806556
TATCAAC13300.033.5747952
GTATAGG856.781537E-427.666141
ACGCAGA15800.027.3666067
GTACTAG1555.0336894E-827.3091581
ATAGACG700.0088306126.856673
TAGTCCG459.5165917E-426.11065332-33
CGCAGAG16700.025.8917628
GTCATAC1101.012095E-425.6540571
GATTGAC950.001303362724.736417
GTATAAG1602.0215903E-623.5162161
GTACATG19300.023.150681
GCAGAGT19000.023.004869
TCATACT1452.5264451E-522.691932
TACATGG19550.022.6007332
Produced by FastQC (version 0.11.2)