FastQCFastQC Report
Sun 14 Apr 2019
SRR6576035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627737
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9020.14369074946992133No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7960.12680469687146048No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7830.124733765892404No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6980.11119306333703446No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6700.10673259661291273No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6320.10067910605874753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.058.7044681
GTATCAA10750.055.17551
TCAACGC13700.042.1931084
ATCAACG13850.041.736153
CAACGCA14200.040.7074365
AACGCAG15000.038.5363736
TATCAAC17300.034.2334482
ACGCAGA18050.032.2850537
TGTACTA600.00416147231.3303855
CGCAGAG18650.031.0092268
GTGTAAT1452.6155249E-829.2185521
ATAGGGT1154.1954736E-628.6060053
GTACATG26050.027.8289741
ACATGGG26050.026.8803883
GCAGAGT21750.026.589529
TACATGG27100.026.3633442
GACTAGG1101.00674115E-425.676911
TAGGTAT2101.4297257E-924.6167325
ATGGGAG6950.023.6668385
ATTATCC3400.023.497793
Produced by FastQC (version 0.11.2)