FastQCFastQC Report
Sun 14 Apr 2019
SRR6576035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627737
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8980.14305353993790393No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8320.13253958265961702No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7920.1261674873394431No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7270.11581283244416053No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7210.11485701814613444No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6770.10784771329394316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGT200.002164353670.484954
GGTATCA3600.069.207231
GTATCAA10400.055.5968131
ATCAACG13750.041.3511663
TCAACGC13800.041.2013444
CAACGCA14400.039.1583025
AACGCAG14650.038.8108256
TAAGACA3650.034.75974
TATCAAC16900.033.657112
ACCTAAG2150.032.7967571
GTCTTAG3150.031.3391231
ACGCAGA18800.029.9935937
CTAAGAC3550.029.120543
GTACTAG650.006146515328.9284231
GTATAGA1303.3221477E-728.9284231
CGCAGAG19900.028.0995278
GACGGTA2550.027.6411558
ACGGTAT2550.027.6389529
GTACATG24250.026.5574861
TAGGTAT2156.548362E-1126.2269555
Produced by FastQC (version 0.11.2)