FastQCFastQC Report
Sun 14 Apr 2019
SRR6576034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709825
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9810.1382030782235058No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7910.11143591730356074No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7910.11143591730356074No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7820.11016799915472124No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7440.10481456697073223No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7190.10129257211284472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11900.060.617191
GGTATCA3950.057.2921941
ATCAACG15900.044.3401533
TCAACGC16200.043.519044
CAACGCA16350.043.119785
AACGCAG16900.041.438376
TATCAAC19650.036.117482
ACGCAGA21550.032.930797
CGCAGAG22500.031.5403798
GTCTTAG4100.028.747991
GCAGAGT25050.028.1420699
ATTATCC3700.027.9462813
GTACATG29500.027.3291051
GGGGATA2104.9112714E-1126.8574665
TACATGG30250.026.1027932
GTGTAGG3750.025.1449091
GTATACA1501.1303564E-625.1449091
AGAGTAC22000.024.45993410-11
CCGACCA5000.024.438579
ACATGGG31200.024.4041393
Produced by FastQC (version 0.11.2)