FastQCFastQC Report
Sun 14 Apr 2019
SRR6576034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709825
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9620.13552636213151129No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8670.12214278167153876No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7930.11171767689219174No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7720.10875920121156622No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7200.10143345190716022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.058.393341
GGTATCA3800.058.1664541
TCAACGC14900.045.1058274
ATCAACG15150.044.3615073
CAACGCA15300.043.926595
AACGCAG16100.042.3277366
TATCAAC18450.036.450042
ACGCAGA20800.032.537347
CGCAGAG21650.031.042818
TAGGACG650.006153641328.9220814
GCAGAGT23450.028.6599929
CTATTAA1853.255991E-1027.9627111
TATAATA1704.0454324E-927.6636452
GTACATG28350.026.8732531
TACTCGT700.00883527226.8562167
CTAAGAC4050.026.6904373
TACATGG28550.025.861392
ACATGGG29050.025.0765883
AGAGTAC21150.023.77695810-11
ACCTAAG2602.7284841E-1123.5140971
Produced by FastQC (version 0.11.2)