FastQCFastQC Report
Sun 14 Apr 2019
SRR6576027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366467
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17480.12792112798918673No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16900.12367660543576976No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15390.11262621051221873No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14670.10735714803211494No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14430.10560079387208034No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14140.10347853259537185No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14140.10347853259537185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20100.064.070231
GGTATCA6650.052.301221
TCAACGC28250.043.5770074
ATCAACG28550.043.119113
CAACGCA29850.041.3986325
AACGCAG30450.040.891516
TATCAAC34550.036.049532
TATAGCG753.2813195E-431.3244425
ACGCAGA40100.031.0510357
CGCAGAG41600.030.0443578
GTACATG55150.029.9984551
TACATGG56000.028.703832
ACATGGG55500.028.4459783
ACCTAAG4400.027.7729741
CTAAGAC8300.027.1730123
GCAGAGT47200.026.3802229
AGAGTAC39950.024.05199610-11
TAGACAG4550.023.7515015
ATTAGAC2052.9962393E-822.9203223
TAAGACA9350.022.6139014
Produced by FastQC (version 0.11.2)