FastQCFastQC Report
Sun 14 Apr 2019
SRR6576026_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576026_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504987
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17300.11495115904655655No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15270.10146267044167159No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15150.10066532136157987No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15130.10053242984823124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23150.055.559461
GGTATCA9100.055.3973161
ATCAACG29700.042.0950783
TCAACGC29650.042.006154
CAACGCA30650.040.7889865
AACGCAG32200.039.263426
TATCAAC38550.033.040842
ACGCAGA41750.030.0560637
ACCTAAG4500.028.2681621
CGCAGAG44550.028.0615228
GTACATG55300.027.5184351
TACATGG56050.027.0014082
CTAAGAC8650.026.6247863
ACATGGG56750.026.585533
GCAGAGT48150.025.7682519
GTATAAT6950.025.7599561
GTCTTAG8700.024.9106431
GTACTAG950.001286542924.7966351
TAAGACA9800.024.4588384
CTATTAA3900.024.1608221
Produced by FastQC (version 0.11.2)