FastQCFastQC Report
Sun 14 Apr 2019
SRR6576026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504987
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18540.12319043287417102No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17510.11634651993671706No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17200.11428670147981344No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16080.10684477673229072No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15950.10598098189552468No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15710.10438628373534123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23600.062.3454741
GGTATCA7600.055.0489461
TCAACGC31050.045.8634874
CAACGCA32200.044.517425
ATCAACG32400.043.807453
AACGCAG33700.042.9543236
TATCAAC40150.035.5926742
CTAGTAC1107.7008735E-834.180893
ACGCAGA42900.033.523567
CGCAGAG44100.032.611368
GCAGAGT49800.028.9731069
GTACATG56250.028.1630691
TACATGG55550.027.7563512
ACATGGG55250.027.6463073
ACCTAAG4950.027.5400941
GTCTTAG8600.026.783681
AGAGTAC42800.025.36613310-11
TTAGGTA4550.024.7905354
AGGTATA4850.024.2261456
CTTAGGT5000.023.4993613
Produced by FastQC (version 0.11.2)