FastQCFastQC Report
Sun 14 Apr 2019
SRR6576023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131587
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16020.14157108556390272No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13440.11877124781391091No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12700.11223175946701403No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12290.10860852943697656No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11640.10286438426740498No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11610.10259926987496322No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11560.10215741255422695No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11540.10198066962593244No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11450.10118532644860713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17900.057.5062831
GGTATCA7050.050.0030331
TCAACGC23800.041.8643884
ATCAACG24100.041.5382653
CAACGCA24900.040.0149545
AACGCAG25550.039.180916
TATCAAC31550.032.1794472
ACGCAGA32750.030.8541057
GTACATG44950.030.010721
CGCAGAG34300.029.5968488
TACATGG46100.028.6503282
ACATGGG46700.027.977813
GCAGAGT37550.027.1603669
GTACTAT1807.492417E-926.1126961
CTTAGGT4250.025.4345873
AGAGTAC32550.024.5461710-11
TAGGTAT4300.024.0458455
GTCTTAG6950.023.6705021
GACCGTA1000.001758946623.4993487
CCCTATA1452.5320413E-522.691032
Produced by FastQC (version 0.11.2)