FastQCFastQC Report
Sun 14 Apr 2019
SRR6576022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131517
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16160.1428171207326094No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA13250.11709943376900214No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13130.11603891059524514No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13060.11542027207722023No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12900.11400624117887756No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12740.1125922102805349No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12330.10896875610353181No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11710.10348938637245396No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11620.10269399399213622No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11480.10145671695608639No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT11470.10136834002493997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.059.477171
GGTATCA5950.052.242391
ATCAACG23250.043.4580353
TCAACGC23250.043.2559054
CAACGCA24350.041.494845
AACGCAG25350.039.6725736
TATCAAC29750.034.4384232
ACGCAGA31700.031.5758977
TACATGG47900.030.612082
GTACATG48300.030.5206131
CGCAGAG33250.030.1225748
ACATGGG49950.029.5425993
GCAGAGT36650.027.5847249
ACCTAAG3950.027.4237441
GTCTTAG5100.026.780821
AGGTATA2500.026.3173756
TCTATAC909.5231214E-426.108513
TAGGTAT2550.025.8013485
ATTATCC4200.024.6165923
GGTACCT8200.024.084618
Produced by FastQC (version 0.11.2)