FastQCFastQC Report
Sun 14 Apr 2019
SRR6576022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131517
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18270.16146465320450334No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15680.1385750280375814No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14570.12876518868032916No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14360.1269092731262544No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13170.11639241831983081No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12240.10817336372321407No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12150.1073779713428963No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12000.10605231737570006No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT11790.1041964018216253No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11610.10260561706098979No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11600.1025172401298434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15550.063.4819531
GGTATCA5100.048.850281
TCAACGC21800.044.4016724
ATCAACG22350.043.5192533
CAACGCA22950.042.1767545
AACGCAG23350.041.655476
TATCAAC30400.032.3186532
GTACATG42550.031.8166391
ACGCAGA30800.031.7322777
CGCAGAG31350.031.1755718
TACATGG43900.030.4112592
ACATGGG44500.029.7767773
GCAGAGT35350.027.6467029
AGAGTAC29500.026.12108610-11
CTAACCC909.531566E-426.1045463
GAGTATA1301.0865744E-525.3113941
CTTAGGT2900.024.304233
ACCTAAG3500.024.1749651
CATGGGG26400.024.0280464
CTAGACT1802.166089E-723.4940914
Produced by FastQC (version 0.11.2)