FastQCFastQC Report
Sun 14 Apr 2019
SRR6576021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243218
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18790.1511400253213837No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17130.13778758029565208No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15590.1254003722597324No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15420.12403295319083217No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13650.10979570759110631No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13640.10971527117528866No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13160.10585432321604095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19600.059.0026971
GGTATCA5900.058.165261
TCAACGC24650.046.7129364
ATCAACG25350.045.2376333
CAACGCA26000.044.6489875
AACGCAG26700.043.4784166
ACGCAGA33150.034.7352647
TATCAAC34450.033.5690232
GTACATG49900.032.5963061
CGCAGAG35750.032.3405278
TACATGG50600.031.6808382
ACATGGG51200.030.7512573
GCAGAGT39750.029.0861329
ACCTAAG4000.028.2061671
GTACTAG856.8076944E-427.6531051
TTAGGTA3100.027.2897054
GTCTTAG5600.026.8630161
TAGGTAT3200.026.4369015
CTTAGGT3200.024.9681853
AGGTATA3300.024.2115736
Produced by FastQC (version 0.11.2)