FastQCFastQC Report
Sun 14 Apr 2019
SRR6576018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780974
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25310.14211324814399312No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG23550.13223101516361271No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21590.12122580116273454No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20420.11465636219282259No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20210.11347723212129991No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19860.1115120153354288No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19570.10988369285570704No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19550.10977139475365726No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18830.10572866307986528No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT18820.10567251402884041No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18710.10505487446756663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28300.064.291851
GGTATCA8500.056.4173431
TCAACGC38300.046.8640564
ATCAACG38500.046.6206053
CAACGCA39250.045.6100545
AACGCAG40850.044.3987276
TATCAAC47450.038.245692
ACGCAGA51550.034.8184937
CGCAGAG55850.032.137758
TTAGGTA5000.030.0715084
GCAGAGT61550.029.2378869
ACCTAAG7900.028.5657441
GTACATG71650.026.3779621
TACATGG71850.026.2391032
CTTAGGT5800.025.9237163
TAGGTAT5700.025.554195
AGAGTAC51650.025.42650810-11
ACATGGG73950.025.4154073
AGGTATA5850.024.095766
CTAAGAC11700.023.6941643
Produced by FastQC (version 0.11.2)