FastQCFastQC Report
Sun 14 Apr 2019
SRR6576017_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576017_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963306
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29520.1503586297805844No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG25090.1277946484144601No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23040.11735307690191953No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22230.11322738279208641No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21880.11144467546067703No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA21450.10925449216780268No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21400.10899981969188706No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20970.10680963639901268No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG20410.1039573046687577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30950.057.8537751
GGTATCA10950.052.4994161
TCAACGC41250.043.182274
ATCAACG42250.042.2725223
CAACGCA43600.041.1781735
AACGCAG45500.039.6652346
TATCAAC52750.033.9471862
ACCTAAG10050.033.7578851
ACGCAGA55500.032.2634777
TAAGACA14250.030.0135084
CGCAGAG59550.029.911398
GTACATG80150.029.3951191
TACATGG81850.028.883552
CTAAGAC14050.028.768913
ACATGGG83700.028.020533
GCAGAGT65500.027.4812589
TATAGGG2750.025.6365642
CCTAAGA15150.025.4391442
GTCTTAG7800.025.3725131
GTACTAG1501.1375887E-625.1308691
Produced by FastQC (version 0.11.2)