FastQCFastQC Report
Sun 14 Apr 2019
SRR6576016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808808
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10830.1339007527126339No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10580.1308097842751308No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10330.12771881583762773No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8920.11028575385011029No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8830.10917300521260918No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8100.10014737737510016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.057.4113661
GGTATCA5400.050.5568731
TCAACGC18500.040.1333854
ATCAACG18900.039.0353663
CAACGCA19250.038.5697485
AACGCAG19400.038.271536
GTACATG34500.034.1088331
TACATGG36000.032.372012
ACATGGG35300.032.08213
TATCAAC23500.031.5943682
ACGCAGA25250.029.2203627
ATGGGAG7300.028.9674385
AACCGTG1503.699097E-828.1967187
CATGGGA16950.027.1691994
ACGGTAC700.00881289726.8706288
CGCAGAG27600.026.7489328
GCAGAGT29050.025.0900469
CATGGGG19200.024.7195534
CTAAGAC5150.024.6363853
GGGCGTA1151.3720317E-424.5340529
Produced by FastQC (version 0.11.2)