FastQCFastQC Report
Sun 14 Apr 2019
SRR6576016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808808
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11330.14008268958764009No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10990.13587897251263586No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10530.1301915905876302No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9070.11214033491261215No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8800.1088020890001088No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8310.10274379086260274No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8180.10113648727510113No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8140.10064193232510064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.065.863841
GGTATCA4550.057.8479041
CTTATCG405.6835235E-446.9868933
ATCAACG21900.045.270483
TCAACGC21900.045.270484
AACGCAG23100.043.122176
CAACGCA22900.043.088425
TATCAAC26150.039.0030942
AGGTATA2950.038.2266246
CTTAGGT3300.037.0199783
TAGGTAT3350.036.467445
ACGCAGA28200.035.323487
GTCTTAG4400.034.182851
CGCAGAG29650.033.5960248
GCAGAGT32300.031.2761089
TTAGGTA3800.030.912434
GTACATG34600.029.7494561
ACCTAAG3950.029.7477361
TACATGG36100.028.5133292
GTATAGT4150.028.3053578
Produced by FastQC (version 0.11.2)