FastQCFastQC Report
Sun 14 Apr 2019
SRR6576014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384868
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5390.14004801646278725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210.1093881538605444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.047.2248841
TCAACGC13300.036.3984954
TATAACG651.4117434E-436.1538472
GTACATG22400.035.8794631
ATCAACG13450.035.6431243
AACGCAG13800.035.079716
TACATGG22950.034.6100232
CAACGCA13800.034.398555
ACATGGG24300.032.6872443
TATCAAC15100.031.7483442
GGGTACC2600.028.9230777
ACGCAGA16100.028.316777
CGCAGAG16300.028.257678
CATGGGA13150.027.8783264
CATGGGT6050.025.6363644
ATGGGAG6450.025.5038765
GCAGAGT18100.025.4475149
CATGGGG9400.025.04
CCTCGAT950.001303009924.73684194
GCCGATG950.001303009924.7368418
Produced by FastQC (version 0.11.2)