FastQCFastQC Report
Sun 14 Apr 2019
SRR6576012_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576012_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948872
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC21100.22236929743948605No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17610.1855887833132393No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14800.15597467308551627No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12980.1367940038277028No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12440.13110303602593396No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11600.12225041944540466No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11260.11866721749614279No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11140.1174025579846386No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10860.11445168579112884No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10600.11171159018286976No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10420.10981460091561347No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10130.10675834042947836No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10060.10602062238110092No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9930.10465057457697138No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9890.10422902140647No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9770.1029643618949658No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9580.10096198433508419No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9510.10022426628670675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.056.5280881
CAACGCA28500.042.547635
ATCAACG28800.041.7780383
GTACTAG5450.040.6544761
AACGCAG33050.038.1121866
GGTATCA9500.036.7210541
TCAACGC34100.035.422514
TATCAAC36400.034.9919742
ACGCAGA35000.034.6441047
CGCAGAG35850.033.6915978
GTATAAG4850.032.0759281
GCAGAGT38350.031.4952759
CTAGGCA8050.029.776584
TAGGCAT7850.028.739035
TAGTACT8200.028.0855394
GTATATA1205.706439E-627.4994371
TTAAGGT2253.6379788E-1227.1557244
GTACATG39650.026.51361
TATTAAG3200.026.4376622
TACATGG40100.026.1373182
Produced by FastQC (version 0.11.2)