FastQCFastQC Report
Sun 14 Apr 2019
SRR6576011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245601
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19330.15518613103232898No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17760.14258177377828055No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14620.11737305927018364No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14280.11464345324064447No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14160.11368006287727772No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13480.10822085081819939No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13440.10789972069707716No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13030.10460813695557405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19300.060.002161
GGTATCA7500.051.4685171
ATCAACG26950.042.022273
TCAACGC27000.041.9444544
CAACGCA28150.040.2309155
AACGCAG29400.038.6818056
TATCAAC33700.033.886952
ACGCAGA37600.030.2458787
GTACATG51750.029.5639211
TACATGG51300.029.4065322
CGCAGAG39150.028.9481288
GGTAATC2300.028.6243348
ACATGGG52100.028.4114953
ACCTAAG7500.026.3619231
CTTAGGT5000.026.315363
GCAGAGT43700.026.0416899
GTCTTAG7300.025.1495841
CTAAGAC10100.024.6590163
TAAGACA10700.024.5937924
TTAGGTA5500.023.9230524
Produced by FastQC (version 0.11.2)