FastQCFastQC Report
Sun 14 Apr 2019
SRR6576010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408223
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20180.1433011674997497No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18750.1331465257988259No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15050.10687227804119091No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14960.10623317471735655No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14920.1059491287956524No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14860.10552305991309616No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14400.10225653181349828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20900.061.5945431
GGTATCA6900.047.1547971
TCAACGC28250.044.761054
ATCAACG28450.044.4479643
CAACGCA29400.043.0101935
AACGCAG31150.040.7447976
TATCAAC35450.035.9364282
GTCTTAG9000.031.9604741
ACGCAGA40100.031.5258227
GTACATG54750.031.522661
TAGGTAT6550.030.8598355
AGGTATA6250.030.836876
CTTAGGT6600.030.6271363
CGTAGAC1859.094947E-1230.4923843
TACATGG56900.030.4030692
ACATGGG56700.030.1786793
CGCAGAG42750.029.7914528
TTAGGTA6800.029.7252834
GTATAGT7450.027.7558738
GCAGAGT46150.027.596639
Produced by FastQC (version 0.11.2)