FastQCFastQC Report
Sun 14 Apr 2019
SRR6576008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532048
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27650.18047737407705242No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24350.15893757897924868No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21870.1427500966027174No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19290.1259098931626163No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18110.11820778461249257No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17290.11285547189122012No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17260.11265965557214917No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17190.11220275082765031No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16680.10887387340344427No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16120.10521863544745334No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15860.10352156068217185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23250.060.453671
GGTATCA8100.048.749341
TCAACGC31500.043.8658144
ATCAACG32700.042.3997883
CAACGCA33100.041.745415
AACGCAG34450.039.97316
TATCAAC40800.034.4497032
ACGCAGA43300.031.6945347
TAGGTAT7900.031.5310335
AGGTATA7700.030.5188876
CGCAGAG45150.030.3958648
GTACATG61300.030.2141381
CTTAGGT7950.030.1503583
ACATGGG61250.029.925953
GTCTTAG10900.029.7575421
TACATGG62100.029.6735122
ACCTAAG10000.029.1451421
TTAGGTA8400.029.0946714
CCCTATA1154.19183E-628.6137432
GCAGAGT49400.027.8759779
Produced by FastQC (version 0.11.2)