FastQCFastQC Report
Sun 14 Apr 2019
SRR6576003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259112
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7560.2917657229306246No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4180.1613202013029115No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3970.1532155978881719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.13546265707493285No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3470.13391892309117293No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3350.12928772113989317No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3190.12311278520485351No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3060.11809564975763377No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3030.11693784926981383No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2980.11500818179011392No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2850.1099910463428942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2820.10883324585507426No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2780.10728951187131434No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2770.10690357837537437No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2770.10690357837537437No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2760.10651764487943438No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2690.10381611040785452No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2660.10265830992003458No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2610.10072864244033469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGA500.001689502437.6456071
GTACCCT801.15629955E-535.2450754
ATGGGTA2450.030.6895925
CATGGGG6600.030.6169384
GTACATG17350.030.1056351
TACATGG17700.029.470462
TAAGGCT804.7746883E-429.3708974
ACATGGG17650.029.0214423
ATACTAG650.006109194428.9581571
TATACGC1951.8189894E-1128.919047
TACGCTA2002.5465852E-1128.2123979
ATATACG2002.5465852E-1128.1960646
ATACGCT2104.9112714E-1126.868958
ATAAGGC909.505468E-426.1074663
TAATATA2357.2759576E-1225.996374
TGGGTAC2750.025.6327846
ATGGGGA3500.025.5107235
CATGGGT4100.025.215994
GTACTTT7000.024.8729881
GTATCAA10050.024.8161321
Produced by FastQC (version 0.11.2)