FastQCFastQC Report
Sun 14 Apr 2019
SRR6576003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259112
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6380.24622557040970702No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3940.15205779740035197No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3750.14472506097749235No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3520.13584859057087284No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3220.12427058569267344No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3010.11616598227793386No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2920.11269258081447406No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2790.10767544536725432No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2740.1057457778875544No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2730.10535984439161443No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2630.10150050943221464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC450.001013790341.7669683
GTATAGG801.1572429E-535.2408831
CATGGGT4550.035.1117944
GTATAGA702.1836681E-433.5627441
ATGGGTA3100.033.346215
CATATAG600.00416035431.3252283
GTCTAAT600.00416035431.3252281
TTATGGC650.00615326628.9155964
TATGCAC650.00615326628.9155963
GTATCAA13100.028.336181
GTACATG20500.027.9634971
TAATACT856.812041E-427.6399064
GAGAATT856.812041E-427.63990694
ACATGGG20950.027.3628483
TACATGG20950.027.1385632
GGGTGTA700.00883476326.8501977
GTATGCG350.00884277526.85019718-19
TAGTAGA700.00883476326.8501974
GCTAGAC909.512078E-426.1043573
GACATAT909.512078E-426.1043571
Produced by FastQC (version 0.11.2)