FastQCFastQC Report
Sun 14 Apr 2019
SRR6576002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798074
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19580.24534065763325208No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17480.21902730824459887No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14500.18168741244546247No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10840.13582700351095262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17150.062.2723121
GGTATCA4800.058.8089261
ATCAACG21700.047.857333
TCAACGC22400.046.7813534
CAACGCA23150.045.265765
AACGCAG23450.044.4862756
TATCAAC25700.041.1426732
CTAGACG608.831467E-539.159283
ACGCAGA27150.038.4236957
TAGGTAT5100.036.8557935
GTCTTAG6150.035.9547231
AGGTATA5150.035.5855186
CGCAGAG29550.035.488698
GGTATAG5400.033.938047
CTTAGGT5650.033.268063
TTAGGTA5850.032.9339564
GCAGAGT32200.032.8601269
ACCTAAG7850.029.9663311
TCTTAGG7800.028.9194322
GTACATG36000.028.8817161
Produced by FastQC (version 0.11.2)