FastQCFastQC Report
Sun 14 Apr 2019
SRR6576002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798074
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18110.22692131306119484No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16820.21075739843673644No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15940.1997308520262532No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11440.14334510333628211No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT8010.10036663266814858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15900.059.703291
GGTATCA4850.059.106071
TCAACGC20000.046.9853444
ATCAACG20100.046.751583
CAACGCA20850.045.069875
AACGCAG21900.042.9089856
TATCAAC23550.040.3092272
ACGCAGA27100.034.675537
CGCAGAG27500.034.342018
TAGGTAT4800.033.2812845
GTACATG34800.033.0849761
GTTCTAA1755.456968E-1232.2245751
ACATGGG35300.032.077813
TACATGG36200.031.5458132
AGGTATA5100.031.3235576
GCAGAGT30500.030.9641089
CTTAGGT5250.030.4286023
TTAGGTA5200.029.817624
GTCTTAG6500.028.919491
GTCTAGA1154.1983258E-628.6051481
Produced by FastQC (version 0.11.2)