FastQCFastQC Report
Sun 14 Apr 2019
SRR6576001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891163
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19730.2213960857890195No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16550.185712378094692No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16460.18470246183919214No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12270.13768524949981092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16500.058.985011
GGTATCA5100.054.3922581
TCAACGC21100.045.8848574
ATCAACG21150.045.7763863
CAACGCA21950.043.8938755
AACGCAG23100.041.9121486
TATCAAC26300.037.0058062
TAACCCG651.4134879E-436.1527445
ACGCAGA29250.033.0998467
GTACATG38800.031.8697951
CGCAGAG31750.030.6415868
TACATGG39650.030.356522
ACATGGG40100.029.7696673
GTCTTAG6000.028.993841
GCAGAGT34350.028.4591059
GTATAGA1853.274181E-1027.9560761
ACCTAAG6750.027.8619461
GGGTACC7150.027.6075527
GTTGCGC700.00883628726.85632394
TATTCCG909.517685E-426.1103155
Produced by FastQC (version 0.11.2)