FastQCFastQC Report
Sun 14 Apr 2019
SRR6575999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241683
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC23800.1916753309822233No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT19410.15632009135987204No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16100.12966272389973932No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15340.12354199904484478No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14760.11887091955032No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14420.11613270053628824No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14280.11500519858933399No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14040.11307233810884099No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14020.11291126640213323No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13710.1104146549481631No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13240.10662946984053094No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12980.1045355376533302No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT12920.10405232253320694No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12460.10034767327892868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29800.049.163151
CAACGCA36800.039.336925
ATCAACG37250.038.7355353
GGTATCA13850.038.0946351
AACGCAG43050.034.9360926
TCAACGC41850.034.477874
ACGCAGA44300.032.99287
TATCAAC45400.032.713632
CGCAGAG45300.032.1607368
GCAGAGT47300.030.7015199
GTACTAG5250.030.50811
GTACATG54650.029.6526721
TACATGG55150.029.145942
ACATGGG54600.028.750893
CGTTAAC1652.8921932E-928.5503621
CATGGGT11600.027.9568734
ATGGGTA9450.027.851835
TAGGCAT7150.027.608375
TGGGTAC8750.027.3942626
GGGTACC8750.027.3920567
Produced by FastQC (version 0.11.2)