FastQCFastQC Report
Sun 14 Apr 2019
SRR6575999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241683
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC24250.19529944438314772No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT19800.15946098964067318No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18070.14552828701045276No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17010.13699148655494195No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC16870.1358639846079877No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16070.12942111633967768No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15750.12684396903235368No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15420.1241862858716758No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT15110.12168967441770565No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14950.12040110076404363No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14770.11895145540367388No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG14690.11830716857684287No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14140.11387769664237973No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14110.11363608908231812No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13550.10912608129450109No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13040.10501875277345345No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12470.10042820913228256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27950.056.8479961
CAACGCA35500.043.027675
ATCAACG35650.042.7147943
GTACTAG6200.040.1850471
GGTATCA11500.037.6071361
ACCGGTC500.001702177137.5995648
AACGCAG41500.037.3730626
TCAACGC41350.036.9403234
ACGCAGA43800.034.981337
TATCAAC46700.033.9229282
CGCAGAG45650.033.5636868
GCAGAGT48600.031.6230939
TAGGCAT7750.031.535125
AGGGCTA6900.029.2895165
GTACATG53750.028.861291
ACATGGG54200.028.355763
TACATGG54450.027.8859182
CTAGGCA9300.027.795384
GTATATA1956.039045E-1026.5178531
ATTTAGG7450.026.5016751
Produced by FastQC (version 0.11.2)