FastQCFastQC Report
Sun 14 Apr 2019
SRR6575998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153385
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15350.13308652358059106No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11980.1038681793156665No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11780.1021341529497956No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11700.10144054240344724No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11620.10074693185709888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18550.062.6707271
GGTATCA5300.053.2828031
TCAACGC25000.045.3012734
ATCAACG25400.044.5878683
CAACGCA25500.044.4130135
AACGCAG26800.042.258656
TATCAAC31900.036.0933842
ACGCAGA33350.034.240797
CGCAGAG37200.030.4642128
GCAGAGT38300.029.7120389
GTACATG44300.029.4298291
ACCTAAG3900.028.9639851
TACATGG46100.027.9319862
ACATGGG45350.027.8745823
AGAGTAC32450.026.15642510-11
CTAAGAC6550.024.3933243
TATACAA1951.7593266E-824.0989865
TAAGACA7350.023.656354
GTATATG2653.6379788E-1123.0892141
ATGGGAG12600.022.004675
Produced by FastQC (version 0.11.2)