FastQCFastQC Report
Sun 14 Apr 2019
SRR6575997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270854
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15290.12031279753614499No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13120.10323766538091708No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT12800.10071967354235813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19600.061.7908821
GGTATCA6200.050.1648641
ATCAACG26350.045.138113
TCAACGC26900.044.2134744
CAACGCA27450.043.668395
AACGCAG29000.041.4964836
TATCAAC33000.036.611932
ACGCAGA36550.032.7883537
CGCAGAG40650.029.8304718
GTACATG49800.029.1453131
GTCTTAG4750.028.7707861
TACATGG50600.028.0582052
GCAGAGT43750.027.931639
ACATGGG52150.027.043973
ATGGGAG13700.025.0479165
AGAGTAC37350.024.97875810-11
CATGGGA23750.022.3666654
GGTATAG2807.6397555E-1121.8198667
AGTACAT33050.021.40257312-13
AGGTATA2458.480129E-921.105516
Produced by FastQC (version 0.11.2)