FastQCFastQC Report
Sun 14 Apr 2019
SRR6575993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574311
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18520.11763876387829342No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18470.1173211646237624No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17810.1131288544639528No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16800.106713349522426No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15990.1015682415990233No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15950.1013141621953985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22550.063.5386471
GGTATCA7250.046.8064161
TCAACGC30600.045.6188354
ATCAACG30950.045.102953
CAACGCA32300.043.2164735
AACGCAG34100.041.073086
TATCAAC39150.035.6560972
ACGCAGA42250.032.9265757
CGCAGAG44400.031.4380118
GTACATG59100.027.8322851
GCAGAGT50150.027.646029
TACATGG59600.026.8127782
ACATGGG60850.025.9530183
CTAGTAC1501.1627253E-625.0673183
AGAGTAC43000.024.86534910-11
GTCTTAG6850.022.7056671
ACCTAAG5200.022.6593571
ATGGGAG16650.021.4533315
GTATATA2206.127084E-821.4233911
TATACCG3151.6370905E-1120.8894334
Produced by FastQC (version 0.11.2)