FastQCFastQC Report
Sun 14 Apr 2019
SRR6575993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574311
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19200.12195811373991543No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19120.12144995493266579No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18680.11865508149279272No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18050.11465333088570173No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17540.11141381848948524No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17140.108873024453237No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16840.10696742892605082No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15770.10017080487908679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22800.059.5899621
GGTATCA7250.056.4145851
TCAACGC30600.043.9269034
ATCAACG31150.043.1526763
CAACGCA32600.041.5203365
AACGCAG33850.040.264786
TATCAAC40400.033.979082
ACGCAGA43350.031.3324767
GTACATG60600.029.1692541
CGCAGAG47750.028.3468678
TACATGG61950.028.0782812
ACATGGG62650.027.6824843
GCAGAGT52600.026.0011889
AGAGTAC43950.023.73996510-11
ACCTAAG5300.023.0625651
TAAGACA11100.022.8642394
CTAAGAC10200.022.5785313
GTCTTAG5550.022.0237121
ATGGGAG17500.021.4851265
CATGGGG38950.021.116244
Produced by FastQC (version 0.11.2)