FastQCFastQC Report
Sun 14 Apr 2019
SRR6575991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371830
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17560.12800419877098476No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14710.1072290298360584No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14280.10409453066342039No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14250.10387584467463169No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13890.10125161280916731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6150.062.768041
GTATCAA21700.062.478791
TCAACGC28350.047.5686234
ATCAACG29550.045.318883
CAACGCA30050.044.879195
AACGCAG30900.043.644656
TATCAAC36100.037.4894182
ACCGTAT1055.0931703E-835.8255848
ACGCAGA39450.033.9473157
CGCAGAG41450.032.330548
TAGGTAT3450.031.3268225
GTACATG51400.030.1323241
GTCTTAG6400.029.422521
ACATGGG52950.028.9296653
TACATGG53750.028.501162
GCAGAGT47100.028.3524179
AGGTATA3700.027.9401366
TTAGGTA4350.027.0048964
GACCGTA1057.4331096E-526.851567
CTTAGGT3950.026.1708223
Produced by FastQC (version 0.11.2)