FastQCFastQC Report
Sun 14 Apr 2019
SRR6575991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371830
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18040.1315031745916039No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16920.12333889767682585No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15150.11043642433829265No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14930.10883272708717552No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14810.10795798313202074No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14490.10562533258494128No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14190.10343847269705429No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14160.10321978670826559No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14090.10270951940109198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20700.057.0001561
GGTATCA7450.056.7883031
TCAACGC27750.042.3311164
CAACGCA28350.041.4352155
ATCAACG28450.041.1244163
AACGCAG29350.040.0234536
GTACTAG851.7529608E-533.1821821
TATCAAC37000.032.0163272
GTACATG49250.030.7342261
ACGCAGA38650.030.3929717
TACATGG50100.029.8374752
ACATGGG50150.029.4199123
CGCAGAG40950.028.8006638
CATATAG1652.9740477E-928.4772933
CTAGACG700.00884804326.8500213
ACCTAAG5550.026.2567731
GCAGAGT46300.025.5732779
TAGGTAT3400.024.8757515
TAAGACA9300.024.7568744
AGGTATA3500.024.1650186
Produced by FastQC (version 0.11.2)