FastQCFastQC Report
Sun 14 Apr 2019
SRR6575990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537926
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19920.12952508768302248No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18580.1208120546762328No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17520.1139196554320559No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17210.11190395376630606No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17010.1106035010787255No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16110.10475146398461303No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15520.10091512855625043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24550.064.529451
GGTATCA7350.050.5265121
TCAACGC31850.048.5475354
ATCAACG32700.047.2855953
CAACGCA33900.045.8890465
AACGCAG35800.043.9787146
TATCAAC43950.036.2593842
ACGCAGA43800.035.8387687
CGCAGAG47000.033.398688
GCAGAGT51500.030.480359
GTACTAG1252.3601751E-730.0856651
GTACATG60400.029.497271
TACATGG60400.029.3416122
ACATGGG60850.028.4228733
TAGGTAT3900.027.7168545
ATTATCC8200.025.7916663
AGAGTAC43950.025.61104810-11
AGGTATA4150.024.914686
CGCATAA950.001304838724.7366794
CTTAGGT4550.023.7573053
Produced by FastQC (version 0.11.2)