FastQCFastQC Report
Sun 14 Apr 2019
SRR6575989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259584
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18480.14671510593973883No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16320.12956658706366547No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14660.1163876327422387No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14660.1163876327422387No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14220.11289441593414969No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13760.1092424165438748No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12770.10138267872567451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.061.8128661
GTATCAA22450.059.461041
TCAACGC30500.042.981314
ATCAACG31100.042.1520923
CAACGCA31700.041.3542635
AACGCAG32800.040.1106346
TATCAAC38200.035.189732
ACGCAGA39300.033.476567
GTACATG55600.031.5329481
TACATGG57000.030.2624782
CGCAGAG43800.029.929918
ACATGGG57900.029.539163
AGGTATA4650.029.303566
ACCTAAG6750.029.2466431
TAGGTAT4700.028.991825
GCAGAGT45750.028.7569129
TTAGGTA5100.028.560574
CTTAGGT5100.028.560573
GTCTTAG6800.028.3403661
CTAAGAC9100.026.333233
Produced by FastQC (version 0.11.2)