FastQCFastQC Report
Sun 14 Apr 2019
SRR6575988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396705
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20570.14727519411758389No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16880.12085587149756033No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16500.1181351824472598No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14740.10553409631955209No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14180.10152465982437237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24700.062.0199971
GGTATCA8500.056.0077061
ATCAACG33400.044.748693
TCAACGC33400.044.748694
CAACGCA34600.043.4683885
AACGCAG35800.042.2739226
TATCAAC42100.036.171172
ACGCAGA43650.034.668917
CGCAGAG48450.031.428228
GTCTTAG7150.029.6655081
GCAGAGT51350.029.5617859
ACCTAAG7550.029.3424381
GTACATG57350.028.1085221
TACATGG58900.026.8914552
ACATGGG58900.026.4126743
TAGGTAT5100.025.804035
CTAAGAC9750.025.5488263
AGAGTAC45600.025.1989610-11
AGGTATA5250.025.0667726
TAAGACA10700.024.158984
Produced by FastQC (version 0.11.2)