FastQCFastQC Report
Sun 14 Apr 2019
SRR6575988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396705
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19420.13904152988641125No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17480.12515169631382433No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16260.1164168525207542No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15550.11133345982150848No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15490.11090387733988208No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14690.10517611091819676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.064.627931
GGTATCA6900.049.737291
TCAACGC31600.044.1726384
ATCAACG32350.043.2938233
CAACGCA32950.042.3628355
AACGCAG34450.040.9275746
TATCAAC39600.035.615122
ACGCAGA41650.033.8524557
GTACATG57700.030.8796081
CGCAGAG46550.030.188088
ACCTAAG8150.029.9953631
TACATGG60600.029.8673342
ACATGGG60300.029.773513
GCAGAGT48950.028.8999949
AGAGTAC42550.026.7300510-11
CTTAGGT4650.026.2787283
CATGGGG34950.024.8776044
CTAAGAC10500.024.6182583
GGGTACC8650.024.4500857
GGTACCT9050.023.8887398
Produced by FastQC (version 0.11.2)