FastQCFastQC Report
Sun 14 Apr 2019
SRR6575986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605563
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13840.22854764904725022No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC12080.19948378616262882No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT11530.19040132901118464No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10500.17339236380029824No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9200.1519247378059756No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8980.14829175494539792No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8680.14333768740824654No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8550.14119092480881426No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC7900.13045711181165295No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7640.12616358661278843No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC7530.1243470951824996No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7290.1203838411527785No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7090.11708112946134423No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7070.1167508582922008No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC6820.11262246867790798No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6720.11097111283219087No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6640.10965002815561717No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC6630.10948489257104545No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6590.1088243502327586No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6520.10766840114075661No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6370.10519136737218093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.10056757100417298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.053.4417761
CAACGCA19500.038.803775
ATCAACG19700.038.171253
GTATAAG4550.035.192291
ACGCAAG702.1842372E-433.5702488
CAGTACG851.7538383E-533.1753049
AACGCAG23850.033.1057556
TATCAAC23250.032.5450942
TCAACGC23750.032.255714
GTGATCG4400.032.0443278
ATAAGGT4700.031.9988753
ACGCAGA23450.031.8666827
TGATCGC4450.031.6842829
CGCAGAG23700.031.5305398
TAAGAGT600.00416015631.3322334
TATTCTC2450.030.6927975
TAAGGTG4750.030.6726074
GCAGAGT25150.029.7126739
ACATGGG25650.029.6831683
GAACCGT953.750275E-529.6831686
Produced by FastQC (version 0.11.2)