FastQCFastQC Report
Sun 14 Apr 2019
SRR6575981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342023
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6590.1926771006628209No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.15993076489008048No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4320.12630729512342737No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4260.124553027135602No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4130.12075211316198034No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3730.10905699324314448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12600.036.618111
CAGCGCA550.002709136334.1952259
GTATAGG702.1599994E-433.6288761
CATGGGT6100.033.12964
GTACATG24750.031.9576241
TACATGG24850.031.7778132
TAGGATC600.004157881331.3318714
ATGGGTA3900.031.331875
ACATGGG24750.030.76223
GGTACCT3400.030.4236938
GGGTACC3400.030.40597
AACGCAG14850.029.7494546
CAACGCA14900.029.6496225
ATCAACG14900.029.33423
TCAACGC15150.029.1603574
CAGTACG650.006136434628.9344229
ATGGGAG4750.027.703975
GTGTTAT1554.9782102E-827.3370251
TATCAAC17000.026.8203162
GTCTAGG1458.492989E-725.9754091
Produced by FastQC (version 0.11.2)