FastQCFastQC Report
Sun 14 Apr 2019
SRR6575981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342023
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6710.1961856366384717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.12250638114980571No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4010.11724357718632958No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3970.11607406519444599No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3610.10554845726749369No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3530.10320943328372652No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3480.10174754329387206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14050.035.123681
CATGGGT5850.034.5461464
GGTACCT2450.034.5247048
GGGTACC2550.031.3279727
TATACTG1701.2187229E-1030.4110075
ACATGGG25450.029.7320693
ATGGGTA3050.029.2779585
TACATGG25950.028.9823232
GTACATG26350.028.8949171
ATCAACG17150.027.9526583
TCAACGC17300.027.7102954
ATATACT1704.0563464E-927.646374
CAACGCA17650.027.4270785
AACGCAG17700.027.3496026
TGGGTAC3150.026.8564766
AACCGCG1406.35584E-726.8525477
CATGGGG8450.026.697564
GTACCTG3350.026.6482589
ATACCGT909.5053413E-426.1104626
TATCAAC19500.025.0697082
Produced by FastQC (version 0.11.2)